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STEMCELL Technologies Inc stemspan tm serum-free expansion medium (sfem) 09650
Stemspan Tm Serum Free Expansion Medium (Sfem) 09650, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stemspan+tm+serum-free+expansion+medium+(sfem)+09650/stemspan+sfem/pmc08520529-336-11-21
Average 90 stars, based on 1 article reviews
stemspan tm serum-free expansion medium (sfem) 09650 - by Bioz Stars, 2026-09
90/100 stars

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Recombinant:

Article Title: Past, present, and future efforts to enhance the efficacy of cord blood hematopoietic cell transplantation
Article Snippet: In our experience, serum-free medium such as SFEM (StemSpan TM Serum-Free Expansion Medium, Catalog #09650, Stemcell Technologies) or Stemline (Stemline II HSC expansion medium, Catalog #S0192, Sigma-Aldrich) with 100 ng/mL SCF, TPO, and Flt3L can efficiently maintain CD34 + HSC and HPC numbers for 7–10 days.

Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity
Article Snippet: Freshly sorted LSK cells (1–2 × 10 4 cells/replicate) were seeded into 200 μL of HSPC culture medium composed of StemSpan TM Serum-free expansion medium (SFEM, #09650, STEMCELL Technologies) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific) and 50 ng/mL recombinant mouse SCF and TPO (STEMCELL Technologies) per well of a 48-well-plate and incubated at 37 °C, with 5% CO 2 and 95% relative humidity.

Article Title: Essential role of zyxin in platelet biogenesis and glycoprotein Ib-IX surface expression
Article Snippet: Collagen (385) was from Chrono-log Corp (Havertown, PA, USA).

Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity
Article Snippet: Freshly isolated or transduced LSK cells were plated into round-bottom 96-well plate as a single cell per well containing 100 μL of single cell colony formation medium composed of StemSpan TM Serum-free expansion medium (SFEM, #09650, STEMCELL Technologies) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific) and 10 ng/mL recombinant mouse SCF, TPO, and IL-3 (STEMCELL Technologies) by BD FACSAria Fusion or FACS MelodyTM.

Incubation:

Article Title: Past, present, and future efforts to enhance the efficacy of cord blood hematopoietic cell transplantation
Article Snippet: In our experience, serum-free medium such as SFEM (StemSpan TM Serum-Free Expansion Medium, Catalog #09650, Stemcell Technologies) or Stemline (Stemline II HSC expansion medium, Catalog #S0192, Sigma-Aldrich) with 100 ng/mL SCF, TPO, and Flt3L can efficiently maintain CD34 + HSC and HPC numbers for 7–10 days.

Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity
Article Snippet: Freshly sorted LSK cells (1–2 × 10 4 cells/replicate) were seeded into 200 μL of HSPC culture medium composed of StemSpan TM Serum-free expansion medium (SFEM, #09650, STEMCELL Technologies) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific) and 50 ng/mL recombinant mouse SCF and TPO (STEMCELL Technologies) per well of a 48-well-plate and incubated at 37 °C, with 5% CO 2 and 95% relative humidity.

Article Title: Essential role of zyxin in platelet biogenesis and glycoprotein Ib-IX surface expression
Article Snippet: Collagen (385) was from Chrono-log Corp (Havertown, PA, USA).

Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity
Article Snippet: Freshly isolated or transduced LSK cells were plated into round-bottom 96-well plate as a single cell per well containing 100 μL of single cell colony formation medium composed of StemSpan TM Serum-free expansion medium (SFEM, #09650, STEMCELL Technologies) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific) and 10 ng/mL recombinant mouse SCF, TPO, and IL-3 (STEMCELL Technologies) by BD FACSAria Fusion or FACS MelodyTM.

Isolation:

Article Title: Past, present, and future efforts to enhance the efficacy of cord blood hematopoietic cell transplantation
Article Snippet: In our experience, serum-free medium such as SFEM (StemSpan TM Serum-Free Expansion Medium, Catalog #09650, Stemcell Technologies) or Stemline (Stemline II HSC expansion medium, Catalog #S0192, Sigma-Aldrich) with 100 ng/mL SCF, TPO, and Flt3L can efficiently maintain CD34 + HSC and HPC numbers for 7–10 days.

Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity
Article Snippet: Freshly sorted LSK cells (1–2 × 10 4 cells/replicate) were seeded into 200 μL of HSPC culture medium composed of StemSpan TM Serum-free expansion medium (SFEM, #09650, STEMCELL Technologies) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific) and 50 ng/mL recombinant mouse SCF and TPO (STEMCELL Technologies) per well of a 48-well-plate and incubated at 37 °C, with 5% CO 2 and 95% relative humidity.

Article Title: Essential role of zyxin in platelet biogenesis and glycoprotein Ib-IX surface expression
Article Snippet: Collagen (385) was from Chrono-log Corp (Havertown, PA, USA).

Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity
Article Snippet: Freshly isolated or transduced LSK cells were plated into round-bottom 96-well plate as a single cell per well containing 100 μL of single cell colony formation medium composed of StemSpan TM Serum-free expansion medium (SFEM, #09650, STEMCELL Technologies) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific) and 10 ng/mL recombinant mouse SCF, TPO, and IL-3 (STEMCELL Technologies) by BD FACSAria Fusion or FACS MelodyTM.

FACS:

Article Title: Past, present, and future efforts to enhance the efficacy of cord blood hematopoietic cell transplantation
Article Snippet: In our experience, serum-free medium such as SFEM (StemSpan TM Serum-Free Expansion Medium, Catalog #09650, Stemcell Technologies) or Stemline (Stemline II HSC expansion medium, Catalog #S0192, Sigma-Aldrich) with 100 ng/mL SCF, TPO, and Flt3L can efficiently maintain CD34 + HSC and HPC numbers for 7–10 days.

Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity
Article Snippet: Freshly sorted LSK cells (1–2 × 10 4 cells/replicate) were seeded into 200 μL of HSPC culture medium composed of StemSpan TM Serum-free expansion medium (SFEM, #09650, STEMCELL Technologies) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific) and 50 ng/mL recombinant mouse SCF and TPO (STEMCELL Technologies) per well of a 48-well-plate and incubated at 37 °C, with 5% CO 2 and 95% relative humidity.

Article Title: Essential role of zyxin in platelet biogenesis and glycoprotein Ib-IX surface expression
Article Snippet: Collagen (385) was from Chrono-log Corp (Havertown, PA, USA).

Article Title: Taz protects hematopoietic stem cells from an aging-dependent decrease in PU.1 activity
Article Snippet: Freshly isolated or transduced LSK cells were plated into round-bottom 96-well plate as a single cell per well containing 100 μL of single cell colony formation medium composed of StemSpan TM Serum-free expansion medium (SFEM, #09650, STEMCELL Technologies) supplemented with 1% Penicillin-Streptomycin (Thermo Fisher Scientific) and 10 ng/mL recombinant mouse SCF, TPO, and IL-3 (STEMCELL Technologies) by BD FACSAria Fusion or FACS MelodyTM.



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STEMCELL Technologies Inc stemspan tm serum-free expansion medium (sfem) 09650
Stemspan Tm Serum Free Expansion Medium (Sfem) 09650, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stemspan+tm+serum-free+expansion+medium+(sfem)+09650/stemspan+sfem/pmc08520529-336-11-21
Average 90 stars, based on 1 article reviews
stemspan tm serum-free expansion medium (sfem) 09650 - by Bioz Stars, 2026-09
90/100 stars
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